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HOME > Protocols > Media and Reagents > NZM Media Recipe

NZM Media Recipe

    1. Dissolve the following in 900ml of H2O
      1. 10g NZ amine
      2. 5g NaCl
      3. 2g of MgSO4-7H2O
    2. Stir until all solids have dissolved
    3. Adjust to pH 7.0 with 5N NaOH (usually requires about 200ul)
    4. Adjust volume to 1 liter with H2O
    5. Sterilize by autoclaving

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