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HOME > Protocols > Cell Biology > Cell Culture > Protocol for Heat Inactivating Serum

Protocol for Heat Inactivating Serum

  1. Place bottles in a 56oC water bath. Make sure the bottles cannot tip over or otherwise become submerged in the water bath. The temperature is critical for complement degredation, so make sure!
  2. Wait until the temperature stabilizes at 56oC (after adding the serum to the water bath)
  3. Gently swirl the bottles every 10 minutes until 30 minutes has elapsed. Do not allow the serum to incubate more than 30 minutes!

Note: Heat inactivation of serum may change the appearance.

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