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HOME > Protocols > > Cell Biology > Cell Culture Protocols > Antibiotics for Cell Culture
Antibiotics for Cell Culture

Antibiotic
Storage Conditions
Working Concentration
Stability in Culture (37oC)
Amphotericin B
-20oC
2.5 ug/ml
~3 days
Ampicillin
4oC
100 ug/ml
~3 days
Chloramphenicol
-20oC
5 ug/ml
~5 days
Ciprofoxacin
-20oC
10 ug/ml
not known
Gentamicin
4oC
50 ug/ml
~5 days
Hygromycin B
4oC
500 ug/ml
not known
Kanamycin
-20oC
100 ug/ml
~5 days
Neomycin
-20oC
50 ug/ml
~5 days
Penicillin
-20oC
100 U/ml
~3 days
Puromycin
-20oC
20 ug/ml
not known
Streptomycin
-20oC
100 ug/ml
~3 days
Tetracycline
-20oC
10 ug/ml
~4 days

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