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HOME > Protocols > Media and Reagents > Thermus Agar Recipe

Thermus Agar Recipe

    1. Combine the following in 900ml water:
      • 0.43g Na2HPO4 ×12 H2O
      • 54mg KH2PO4
      • 0.2g MgCl2 × 6 H2O
      • 40mg CaSO4 × 2 H2O
      • 0.5ml Fe-citrate solution (0.01 M)
      • 1ml Trace elements solution (see below)
      • 0.1g Nitrilotriacetic acid
      • 2.5g Tryptone
      • 2.5g Yeast extract
      • 15g Agar
    2. Adjust pH to 7.0 - 7.2
    3. Adjust to 1000ml with water
    4. Autoclave or filter sterilize.

Trace elements solution:

  1. Combine the following in 10 ml distilled water:
    • 45mg CoCl2 × 6 H2O
    • 25mg Na2MoO4 × 2 H2O
    • 25mg CuSO4 × 5 H2O
    • 0.5g H3BO3
    • 0.5g ZnSO4 × 7 H2O
    • 2.28g MnSO4 × H2O
    • 0.5 ml H2SO4
  2. Autoclave or filter sterilize.

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