Home | Jobs | News & Articles | Job Advice | Search | Protocols | Fun | @RealLabRat
Lab Rat with DNA

Post your resume
Candidates - post your resume

Search job listings
Candidates - search biotech jobs

Post job openings
Employers - post job openings

HOME > Protocols > Media and Reagents > 1X Phosphate Buffered Saline Tween-20 (PBST) Recipe

1X Phosphate Buffered Saline Tween-20 (PBST) Recipe

  1. Dissolve the following in 800 ml of distilled H2O
    • 8g of NaCl
    • 0.2g of KCl
    • 1.44g of Na2HPO4
    • 0.24g of KH2PO4
    • 2ml of tween-20
  2. Adjust pH to 7.2
  3. Adjust volume to 1L with additional distilled H2O
  4. Sterilize by autoclaving

PBST is used in several protocols, most commonly as a wash solution in ELISA assays.

theLabRat.com 2005. All Rights Reserved.