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HOME > Protocols > Media and Reagents > Halobacterium Agar Recipe

Nitrosomonas europaea Medium Recipe

    1. Combine solutions 1, 2 and 3. Dispense aseptically into desired aliquots.

Solution 1

  1. Combine the following in 1.2 L distilled water:
    • 4.95g (NH4)2SO4
    • 0.62g KH2PO4
    • 0.27g MgSO4 x 7 H2O
    • 0.04g CaCl2 x 2 H2O
    • 0.5ml 30mM FeSO4/50mM EDTA
    • 0.2mg CuSO4 x 5 H2O
  2. Filter sterilize.

Solution 2

  1. Combine the following in 300ml distilled water:
    • 8.2g KH2PO4
    • 0.7g NaH2PO4
  2. Adjust pH to 10.4 with 10M NaOH.
  3. Filter sterilize.

Solution 3

  1. Combine the following in 12ml distilled water:
    • 0.6g Na2CO3 anhydrous
  2. Filter sterilize.

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