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HOME > Protocols > Media and Reagents > 10X Hellings Recipe

10X Hellings Recipe

  1. Dissolve the following in 800ml distilled H2O
    • 60.55g Tris
    • 16.5g NaAc (anhydrous)
    • 6.95g EDTA
  2. Adjust pH to 8.05 with glacial acetic acid
  3. qs to 1000ml with distilled H2O
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