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HOME > Protocols > Media and Reagents > Halobacterium Agar Recipe

Halobacterium Agar Recipe

    1. Dissolve the following in 900ml of distilled water
      • 7.5g Casamino acids
      • 10g Yeast extract
      • 3g Na3-citrate
      • 2g KCl
      • 20g MgSO4 x 7 H2O
      • 50mg FeSO4 x 7 H2O
      • 0.2mg MnSO4 x H2O
      • 250g NaCl
    2. Adjust pH to 7.4
    3. Add 20g Agar
    4. Bring to 1000ml with distilled water
    5. Autoclave to sterilize

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