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HOME > Protocols > Media and Reagents > ELISA Coating Buffer Recipe

ELISA Coating Buffer Recipe

  1. Dissolve 5.3g of Na2CO3 in 900ml distilled H2O.
  2. Dissolve 4.2g of NaHCO3 in the solution from step 1.
  3. Dissolve 1g sodium azide in the solution from step 2.
  4. pH to 9.6.
  5. Adjust volume to 1L with additional distilled H2O.

Note: For coating step add 0.1 to 1ug/ml anti target antibody to buffer and incubate at 4oC for 12-24 hours.

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