Home | Jobs | News & Articles | Job Advice | Search | Protocols | Fun | @RealLabRat
Lab Rat with DNA

Post your resume
Candidates - post your resume

Search job listings
Candidates - search biotech jobs

HOME > Protocols > Media and Reagents > ELISA Blocking Solution Recipe

ELISA Blocking Solution Recipe

  1. Dissolve 5.3g of Na2CO3 in 900ml distilled H2O
  2. Dissolve 4.2g of NaHCO3 in the solution from step 1
  3. Dissolve 1g sodium azide in the solution from step 2
  4. Dissolve 5g BSA in solution from step 3
  5. Alternatively you can also use 50g non-fat dry milk for blocking solution
  6. pH to 9.6
  7. Adjust volume to 1L with additional distilled H2O

theLabRat.com 2005. All Rights Reserved.