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HOME > Protocols > Media and Reagents > Bifidobacterium Medium Recipe

Bifidobacterium Medium Recipe

  1. Dissolve the following in 1 L of distilled water to make Salt Solution:
    • NaCl 2.00 g
    • NaHCO3 10.00 g
    • KH2PO4 1.00 g
    • K2HPO4 1.00 g
    • MgSO4 x 7 H2O 0.50 g
    • CaCl2 x 2 H2O 0.25 g
  2. Dissolve the following in 950 ml of distilled water:
    • Resazurin (25 mg/100 ml) 4 ml
    • Salt solution (see above) 40 ml
    • NaCl 5.0 g
    • Tween 80 1.0 ml
    • MnSO4 x H2O 0.05 g
    • MgSO4 x 7 H2O 0.2 g
    • K2HPO4 2.0 g
    • Glucose 10.0 g
    • Bacto Soytone 5.0 g
    • Yeast extract 5.0 g
    • Meat extract 5.0 g
    • Casein peptone, tryptic digest 10.0 g
  3. Boil medium and cool under CO2.
  4. Add Cysteine-HCl x H2O 0.5 g to medium
  5. Adjust pH to 6.8 using 8 N NaOH.
  6. Distribute under N2 and autoclave to sterilize.


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