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HOME > Protocols > Media and Reagents > Best's Carmine Stain Recipe

Best's Carmine Stain Recipe

  1. Add the following to 75ml of distilled water
      • 2.5g Carmine
      • 1.25g Potassium carbonate
      • 6.25g Potassium chloride
  2. Heat to solution from step 1 to a gentle boil until dissolved
  3. Cool solution from step 2
  4. Add 25ml of concentrated ammonia to the solution from step 3
  5. Store at 4oC

Best's Carmine stain is used for selectively staining glycogen.

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