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HOME > Protocols > Media and Reagents > Azotobacter Agar Recipe

Azotobacter Agar Recipe

    1. Dissolve the following in 700ml of distilled water
      • 15g Agar
      • 5g CaCO3
      • 10mg FeSO4 × 7 H2O
      • 0.1g KH2PO4
      • 0.9g K2HPO4
      • 5mg Na2MoO4 × 2 H2O
      • 0.1g MgSO4 × 7 H2O
      • 0.1g CaCl2 × 2 H2O
    2. Bring to 900ml with distilled water
    3. Autoclave to sterilize
    4. Add 50ml sterile 100 g/L manitol
    5. Add 50ml sterile 100 g/L glucose

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