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HOME > Protocols > Media and Reagents > Alicyclobacillus Agar Recipe

Alicyclobacillus Agar Recipe

  1. Dissolve the following in 500ml of distilled water to make trace elements solution:
    • Na2MoO4 x 2 H2O 0.03 g
    • NiCl2 x 6 H2O 0.02 g
    • CuCl2 x 2 H2O 0.01 g
    • CoCl2 x 6 H2O 0.20 g
    • H3BO3 0.30 g
    • MnCl2 x 4 H2O 0.03 g
    • ZnSO4 x 7 H2O 0.10 g
  2. Dissolve the following in 1L of distilled water to make Solution A:
    • KH2PO4 3.0 g
    • Glucose 5.0 g
    • Yeast extract 2.0 g
    • (NH4)2SO4 0.2 g
    • MgSO4 x 7 H2O 0.5 g
    • CaCl2 x 2 H2O 0.25 g
  3. Dissolve the 12g of agar in 500ml to make Solution B.
  4. Autoclave solutions separately.
  5. To make final agar combine solution A with solution B and aseptically add 1ml of trace elements solution.


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