Alcoholic Bouin’s Fixative Recipe

1. Stock solution (stable for approximately 1 year)
   • Measure 750ml of 80% ethanol
   • Add 300ml of Formaldehyde
   • Slowly add 5g of Picric acid
2. Working solution (prepare right before use)
   • 70ml of stock solution
   • 5ml of acetic acid

• Caution: Picric acid can become explosive if allowed to dry out and is toxic through skin
  exposure. Formaldehyde is a carcinogen. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.