Home | Jobs | News & Articles | Job Advice | Search | Protocols | Fun | @RealLabRat
Lab Rat with DNA

Post your resume
Candidates - post your resume

Search job listings
Candidates - search biotech jobs

HOME > Protocols > Media and Reagents > ACD Blood Anticoagulant Recipe

ACD Blood Anticoagulant Recipe

I have been there before. You need to take some blood for your protocol and you have no anticoagulant tubes. Here is a quick protocol to make ACD anticoagulant.

  1. Dissolve 1.32g of sodium citrate in 85ml of distilled water
  2. Dissolve 0.48g of citric acid in the solution from step 1
  3. Dissolve 1.47g of dextrose in the solution from step 2
  4. Add distilled water to 100ml
  5. Filter sterilize through 0.2um filter
  6. Use 0.25ml of solution for 1ml of blood
theLabRat.com 2005. All Rights Reserved.