Blood Anticoagulant Recipe
I have been there before. You need to take some blood
for your protocol and you have no anticoagulant tubes.
Here is a quick protocol to make ACD anticoagulant.
Dissolve 1.32g of sodium citrate
in 85ml of distilled water
- Dissolve 0.48g of citric acid in the solution from
- Dissolve 1.47g of dextrose in the solution from
- Add distilled water to 100ml
- Filter sterilize through 0.2um filter
- Use 0.25ml of solution for 1ml of blood