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HOME > Protocols > Media and Reagents > 40% Acrylamide Recipe

40% Acrylamide Recipe

  1. Dissolve 380g of acrylamide and 20g of N,N'-methylbisacrylamide in 600ml of H2O.  Heating may be necessary to dissolve the acrylamide.
  2. Adjust the volume to 1L with H2O.
  3. Sterilize the solution by filtration (0.45 micron pore size).
  4. Check the pH (should be 7.0 or less).
  5. Store in dark bottles at room temperature.

Caution: Acrylamide is a neurotoxin!  Use extreme care when handling solids and solutions containing acrylamide and bisacrylamide!  Wear a mask and gloves when weighing out solid acrylamide!

Related reagents: Acrylamide (30%)

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