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HOME > Protocols > Media and Reagents > LB Broth Media Recipe

LB Broth Media Recipe

  1. Add the following to 800ml H2O
      • 10g Bacto-tryptone.
      • 5g yeast extract.
      • 10g NaCl.
  2. Adjust pH to 7.5 with NaOH.
  3. Adjust volume to 1L with dH2O
  4. Sterilize by autoclaving

Related reagents: LB Agar


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