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HOME > Restriction Enzymes > SphI Restriction Enzyme
SphI

Recognition Sequence:
...GCATGC...
...CGTACG...

After cut:

...GCATG C...
...C GTACG...

Reaction Conditions:
37oC

Compatible ends (at this website):
BbuI, PaeI

Heat Denaturation:
65°C for 20 minutes

Source:
Streptomyces phaeochromogenes

Suppliers:
American Allied Biochemical, Amersham Biosciences, CHIMERx, EURx Ltd., Invitrogen, Minotech Biotechnology, New England Biolabs, Nippon Gene Co., Ltd., Promega, Qbiogene, Roche Applied Science, SibEnzyme Ltd., Sigma, Takara Bio Inc., Toyobo Biochemicals


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