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SpeI
| Restriction Site Sequence: |
...ACTAGT...
...TGATCA... |
|
Restriction Site Sequence After Cut:
|
...A
CTAGT...
...TGATC A... |
| Restriction enzyme reaction conditions: |
37oC |
| Compatible
ends (at this website): |
AhlI,
BcuI |
| Heat
Denaturation: |
SpeI can be denatured by heating at 65°C for 20 minutes |
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