Styrene Mineral Salts Medium Recipe

1. Dissolve the following in 800ml of distilled water
• MnCl₂ x 2 H₂O 1.0 mg
• CoCl₂ x 6 H₂O 0.4 mg
• CuSO₄ x 5 H₂O 0.2 mg
• Na₂MoO₄ x 2 H₂O 0.2 mg
• FeSO₄ x 7 H₂O 5.0 mg
• CaCl₂ x 2 H₂O 1.0 mg
• ZnSO₄ x 7 H₂O 2.0 mg
• EDTA 10.0 mg
• MgCl2 x 6 H2O 0.1 g
• (NH₄)2SO₄ 2.0 g
• NaH₂PO₄ x 2 H₂O
• 0.85 g K₂HPO₄ 1.55 g
2. q.s. to 1L with distilled water
3. Autoclave to sterilize.